![]() ![]() Blots with serial dilution of mouse heart tissue extract were probed for Stat3 Mouse mAb and p38 MAPK Rabbit Ab, detected with IRDye 800CW Goat anti-Mouse IgG and IRDye 680LT Goat anti-Rabbit IgG, and scanned together on an Odyssey CLx Imaging System. Figure 1: Western blot protocol demonstrating the comparison of Intercept Blocking Buffer to Odyssey Blocking Buffer. Intercept Blocking Buffer is ready to use, so you can block and move on with your research. With a convenient 1x formulation, you won’t need to dilute Intercept Blocking Buffers. Intercept non-mammalian protein-based blocking buffers provide the same highly efficient blocking with high signal-to-noise ratio in immunoassays as Odyssey Blocking Buffers, but in a new, cost-effective formula. Several options of Intercept Blocking Buffer are available to provide one that best suits your experiment. Blocker choice may affect antibody specificity and non-specific binding.No single blocking buffer selection is suitable for ALL antigen-antibody pairs.Excessive blocking may cause loss of blotted proteins 1 or mask antibody:antigen interactions.Insufficient blocking yields high background and reduced signal-to-noise ratios.Choosing the right blocker is important because: Blockers enhance sensitivity by reducing background interference, increasing signal-to-noise ratio, and promoting specific binding of the primary antibody while minimizing non-specific interactions. Choose the Right Blocking Buffer for Your Applicationīlocking buffer choice is important for immunoassay success. Because Intercept Blocking Buffers contain no sodium azide, they can provide the same excellent performance for chemiluminescent Western blot detection, as well. Intercept Blocking Buffers can be used to dilute HRP-conjugated secondary antibodies and can be used in the blocking step prior to incubating the blot with HRP-conjugated secondary antibodies. They provide excellent performance with low variability and low background for quantitative Western blots and other immunoassays.īecause no single blocking buffer is ideal for every experiment, several varieties of Intercept Blocking Buffer are available. Intercept ® Blocking Buffers are optimized for use with IRDye ® products and other near-infrared fluorophores. LI-COR offers several blocking buffers for use with the Odyssey ® Imaging Systems. ![]()
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